Composite

Part:BBa_K3748068:Design

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pHHF1-AO-tTDH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
    Illegal BglII site found at 2191
    Illegal XhoI site found at 2218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1758
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Golden Gate Assembly


Source

MoClo Kit and Uniprot

References

Lee, M. E., Deloache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol., 4, 975–986. https://doi.org/10.1021/sb500366v

https://www.uniprot.org/uniprot/P0C1B3 - Alpha amylase A. oryzae